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ClinicalWannamaker and Laboratory Standards Institute. All rights reserved. Licensed to: Cameron This document is protected by copyright. CLSI order # 71377, id # 477046, Downloaded on 12/11/2009. 49 Number 12 GP2-A5 Appendix K. Sample Computer Procedure Signing On To and Navigating IDX Document #/version # Effective Date: mm/dd/yy Signing On to and Navigating the IDX System Purpose This procedure provides instructions for “signing on” to the IDX system. • Do not share your password with another employee – passwords are confidential information.

Materials Reagents • Isotonic saline • Anti-D Reagent • IgG Antiglobulin Reagent • IgG Sensitized Red Cells Quality control Reagents must be evaluated each day of use with appropriate controls. Verify that reagent QC has been performed. If not, see Procedure ID. ” Procedure NOTE: If the original direct examination with the anti-D was performed by tube method, the same tube may be used for the weak D examination, provided the manufacturer’s directions so state. In this case, proceed directly to step 4, after recording the original anti-D tube examination as negative.

Wash well in running water. Rinse in 95% alcohol. Stain one minute in eosin; make sure stain covers slides completely. Wash well in running water. Dehydrate in 95% alcohol and three changes of absolute alcohol, ten dips each. Clear in three changes of xylene, ten dips each. Cell Components Nuclei Cytoplasm and intercellular substances Cells with much RNA or acid mucopolysaccharide Expected Color Blue Shades of pink and red Purplish Related Procedures • Iodine Solution Preparation Procedure • Sodium Thiosulphate – 3% Aqueous Preparation Procedure • Harris Hematoxylin Stain Preparation Procedure • Acid Alcohol Preparation Procedure • Scott’s Tap Water Substitute Preparation Procedure • Eosin Stain Preparation Procedure Reference Bancroft JD, Gamble M.

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