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A good, recent example is the CP of human pluripotent (embryonic and induced alike) stem cells (hESc’s and iPSC’s respectively). , 2007; Ware & Baran, 2007], see Chapter by Martin-Ibanez [Martin-Ibanez, 2012] in this Book). , 2004] despite what is claimed otherwise by the authors. However, the strengths of SF freezing can be its weaknesses as well: it indeed needs elaborative protocols that have to be developed for each new species of cells separately. Secondly, it is difficult to implement for CP of large chunks if tissues, and especially if we are talking about CP of a whole organ.

Katkov I. , Critser J. K. &Mazur P. (1998). " Cryobiology 37(4): 325-38. Katkov I. , Isachenko V. &Nawroth F. (2003). "Why can we vitrify mammalian spermatozoa without cryoprotectants? " Cryobiology 47: 267. Katkov I. I. &Levine F. (2004). " Cryobiology 49(1): 62-82. Katkov I. , Kim M. , Lulat A. -M. , Mackay A. M. &Levine F. (2006). " Int J Refrigeration 29(3): 346-57. Katkov I. , Isachenko V. &Isachenko E. (2007). Vitrification in Small Quenched Volumes with a Little Amount of or without Vitrificants: Biophysics and Thermodynamics.

2003). " Reprod Biomed Online 6(2): 191-200. , Katkov I. , Dessole S. &Nawroth F. (2004a). " Hum Reprod 19(4): 932-9. , Isachenko V. &Katkov I. I. (2007). Cryoprotectant-Free Vitrification of Spermatozoa. In: Vitrification in Assisted Reproduction: A User's Manual and Troubleshooting Guide. Eds:M. J. Tucker and J. Liebermann. Oxon, UK, Infroma Healthcare Medical Books: 87-105. , Weiss J. , Katkov I. , Lulat A. , Risopatron M. J. &Sanchez R. (2008). " Reproduction 136(2): 167-73. , Katkov I. I.

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