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By Gerrit Eggink, National Research Council Canada

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Extra resources for 1996 International Symposium on Bacterial Polyhydroxyalkanoates

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Zuber and Ms. P. Köhler-Sauer ran the Symposium Office with the support of Ms. D. Quednau from EAWAG, Dübendorf. In Davos we were helped by Mr. R. Jäggi from Cresta Hotels, Ms. R. Wenger from the Kongress-Zentrum, and Mr. R. Frey from the Kongress Hotel. FOR THE SCIENTIFIC COMMITTEE OF ISBP96 BERNARD WITHOLT Page 1 Assessment of the Biodegradability of Polymers Under Anaerobic Conditions Morton A. Barlaz,1 Brenda B. Ress and Philip P. Calvert Department of Civil Engineering Box 7908 North Carolina State University Raleigh, North Carolina 27695-7908 United States Abstract The objective of this research was to develop and validate a method to quantify biodegradability under simulated landfill conditions.

Coli indicated that the majority of cPHB in cells is covalently bound to proteins (Huang and Reusch Page 13 1996); we decided to reexamine the cPHB content of eukaryotes. This time, both chloroform-soluble cPHB and total cPHB were determined. As shown in Table 2, the protein fraction of Table 2. 6 * Samples tested were acetone powders (Sigma). cPHB was determined as previously described (Huang and Reusch 1996). Briefly, for measurement of total cPHB, 10 mg of dry sample were heated in conc. sulfuric acid at 120°C for 40 min.

1994; Huang and Reusch 1996). Finally, one must consider the role played by the highly conspicuous inclusion bodies of gPHB in diverting attention from the existence of cPHB. Their prominence and abundance sustained the view that only bacteria which contain these inclusions are capable of PHB synthesis, and the discovery of minute amounts of cPHB in eukaryotic cells, or even in bacteria with no visible granules, could be attributed to contamination. The conviction that synthesis of PHB is restricted to only a few bacterial species is remarkable in view of the fact that the precursor, R-3)-hydroxybutyryl-CoA, is a common metabolic intermediate, and the polymerization of a CoA ester is a common anabolic reaction.

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